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Fisher Scientific sds hypotonic buffer with aprotinin
Sds Hypotonic Buffer With Aprotinin, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/hypotonic+buffer/buffer+tris/pm41453233-54-3-12
Average 86 stars, based on 1 article reviews
sds hypotonic buffer with aprotinin - by Bioz Stars, 2026-10
86/100 stars

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Incubation:

Article Title: A biomechanical characterisation of acellular porcine super flexor tendons for use in anterior cruciate ligament replacement: Investigation into the effects of fat reduction and bioburden reduction bioprocesses
Article Snippet: For fat reduction, the tendons were then subjected to either 3×1 h washes with acetone (VWR International) at room temperature, or 1×1 h with chloroform–methanol (2:1; v/v [VWR, Atom Scientific]) and 1×30 min wash with methanol (Atom Scientific) at room temperature. .. Tendons were subsequently washed five times for 5 min in PBS plus 10 KIU ml −1 aprotinin (NHS Supplies, Leeds, UK) at room temperature, before being transferred to individual pots containing 100 ml hypotonic buffer (10 mM tris, 2.7 mM EDTA [Fisher Scientific], 10 KIU ml −1 aprotinin [NHS Supplies, Leeds, UK]), and incubated at 37 °C. .. After 24 h the solution was changed to hypotonic buffer containing the ionic surfactant sodium dodecyl sulphate (0.1%; w/v SDS [Sigma], 10 mM tris, 2.7 mM EDTA [Fisher Scientific], 10 KIU ml −1 aprotinin [NHS Supplies, Leeds, UK]) for 24 h. This was repeated twice.



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